Dear Plant Lipid Researchers,
The lipid researchers at Kansas State University plan to establish a
"Lipidomics Research Center" in 2003. The Center will have capability
to provide sensitive, high throughput, and quantitative electrospray
ionization tandem mass spectrometry-based lipid profiling of complex
plant lipids.
Profiling will be performed on extracts and generally will not
require any chromatographic procedures. Lipids are speciated to total
acyl carbon number and total number of double bonds and quantified in
comparison to a mixture of internal standards, which we will provide.
The classes of lipids that currently can be profiled are
phosphatidylcholines, phosphatidylethanolamines,
phosphatidylglycerols, phosphatidylinositols, phosphatidic acids,
phosphatidylserines, lysophosphatidylcholines,
lysophosphatidylethanolamines,
lysophosphatidylglycerols, monogalactosyldiacylglycerols,
digalactosyldiacylglycerols, and sulfoquinovosyldiacylglycerols.
A description of the technique and a sample of data obtained by this
method can be found in Welti at al. (2002) J. Biol Chem. 277,
31994-32002;
http://www.jbc.org/cgi/reprint/277/35/31994.pdf
and also at our website www.ksu.edu/lipid/lipidomics.
We are writing to gauge interest in use of the complex lipid
profiling technology provided by the Lipidomics Research Center.
Please reply and let us (welti@... or wangs@...) know
if you are interested in utilizing this facility.
Sincerely,
Ruth Welti
Xuemin (Sam) Wang
Xuemin Wang
Professor
Department of Biochemistry
Kansas State University
Manhattan, KS 66506
phone: (785) 532-6422
fax: (785) 532-7278
email: wangs@...
http://www.ksu.edu/bchem/fac/xw/main2.html
Ruth Welti, Ph.D.
Division of Biology
Ackert Hall
Kansas State University
Manhattan, KS 66506-4901
(785) 532-6241
http://www.ksu.edu/biology/bio/faculty/welti/welti.html
fax (785) 532-6653
welti@...