As you need unsaturated phospholipids for your
experiments, the best way is to prepare them from
animals previously injected with radioactive
precursors. As an example, you may read the ancient
paper by Zborovsky et al., (Biochim Biophys Acta 1977,
497, 183) and the book of Bergelson (Lipid biochemical
preparations, Elsevier 1980) concerning the
preparation of phosphatidylcholine from rats injected
with C14 labelled choline chloride. This approach
which is the cheapest may be extended to any
phospholipid group.
Leray Claude
http://www.cyberlipid.org




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