Dear all
I want to separate phospholipids by solid phase extraction on
aminopropyl columns. Does anyone know of a method for isolating the
extract into the individual classes, LPC, PC, PE, PI, PS and SPH? At
the moment I am eluting the neutral PLs in MeOH, as Kaluzny et al.
1985, and separating these by thin-layer chromatography. I was
hoping to separate PI and PS on the column using different acid
concentrations, does anyone know their pKas?
Many thanks for any information given.
Regards
Simon Dyall