Hello everybody,

I am not very confident with the furtherance of previous contacts I
have made with lipid specialists... this site might then be very
useful to solve my problem.

I work in a lab dedicated to cell biology and we are far more
comfortable with proteins there than with lipids there.
It turns out that we have produced monoclonal antibodies able to
interfere with binding of (what looks very much like a) GSL at the
surface of embryonic stem cells with a (yet to be identified)
membrane receptor present at the surface of cells of a feeder layer.

Co-cultures always result in cell cycle exit for the ES cells unless
one of our monoclonal is included before, during or long after co-
culture onset. We are then very excited about identifying this
receptor/ligand system able to command proliferation or cell cycle
arrest at will.

Despite proficient technical information available from Dr C. Leray's
web site:

www.cyberlipid.com

and as we are a small group with lots of things to do, we think it is
more efficient to collaborate with specialists than to try to become
one! For this reason, we are now seeking a laboratory willing to
collaborate with us in the formal identification of "our" GSL. To
this end we would of course provide all necessary reagents as well as
manpower on site in order to achieve rapid identification by means of
HPTLC-immunostaining based techniques.

On another hand, should the work require mass cell culture, solvent
extractions and large scale preparative chromatographies we would
obviously have to discuss further the terms of said collaboration.

We are looking eagerly forward to hearing from you

Thanks for this nice forum

Jopadove et al.