Hello,

I can give you the Rf values I had with the first
solvent system according to the Heape's method with a
7.5 cm running distance on HPTLC plates :

PE: 0.56, PI: 0.39, PS: 0.27, SM: 0.07, PA: 0.49, CL:
0.45, PG: 0.53

It must be noticed that these values are not
definitive because of the variability of the
separation parameters.
Leray Claude





--- gonzalo obal <gonchi3001@...> a écrit :
>
> Hi, everyone.
>
> I´m looking for Rf values of lipid classes separated
> by HPTLC using silica gel plates using two
> solvent-step:
> Fist for PL:
> Methylacetate/isopropanol/chloroform/methanol/KCl
> (0.25%) (25:25:25:10:9 v/v).
> And then for NL:
> Hexane/diethyleter/acetic acid (80:20:1) (v/v).
> Has anyone this information? Thanks in advance.
>
>
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