Dear collegues,
Does anybody know anything about the thechnique called: "freezing-out
technique". I encountered this technique in many papers but found no
references on the literature.
Basically, when I stick my acetone extract of a freeze-dried tissue
(or a wet tissue) on the refridgerator (-20 degrees C), I see a fat
precipitation - floculation appearing on my tubes.
Any idea which fat precipitate? Triglycerides? Polar fat?
And what is the physico-chemical process taking place on my tube at
this temperature?
Any help, references on that topic would be appreciated.
Maxime