Hi Max,
the fat precipitated may be a triglyceride containing saturated fatty
acids as major fractions. Bcaz, we have come across a similar type of problem
where we found that a triglyceride rich in saturated fatty acid was precipitated
out.
max51692000 <max51692000@...> wrote:
Dear collegues,
Does anybody know anything about the thechnique called: "freezing-out
technique". I encountered this technique in many papers but found no
references on the literature.
Basically, when I stick my acetone extract of a freeze-dried tissue
(or a wet tissue) on the refridgerator (-20 degrees C), I see a fat
precipitation - floculation appearing on my tubes.
Any idea which fat precipitate? Triglycerides? Polar fat?
And what is the physico-chemical process taking place on my tube at
this temperature?
Any help, references on that topic would be appreciated.
Maxime







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