Hi friend,

The new spot developed may be a Fatty alcohol, why not
silylate it and see whether it is an alcohol or not.


gonzalo obal <gonchi3001@...> wrote:

Dear Friends,

The analysis by HPTLC of a parasite lipid extract
shows a band migratin between FAMEs (used as a
standard of migration) and sterol esters. The
migration pattern (in hexane/diethylether/acetic acic
80/20/1)is follows: TAGs - FAMEs - Unknown - Sterol
sters - Hidrocarbons; all bands resolved clearly. I
have checked a lot of information (generally
considered comprehensive) bu i could found any
reference about the kind of lipid class it could be.
Generally, available information reports TAGs, FAMES,
and SS, but no data of lipid classes migrating
slightly further than FAMEs. This lipid component is
preseumed to be biologically relevant and not a
contamintant: it is seen in several independent
extracts but not in controls. Has anyone any idea,
about it? Thanks in advance.

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