To store lipids dissolved in any solvent you must use only Teflon
covered septa, of course Teflon facing the solvent and the silicone
rubber on the upper side. It is convenient to use screw caps with an
hole to verify systematically the orientation of the liner when you warm
or store your vials. All other materials are known to free some
chemicals which give later on spurious spots and peaks on chromatograms.
We use several times the same septa (after washing some minutes in a
sonicator filled with detergent and after rinsing with pure water) until
the Teflon liner is finally separated from the silicone part. All
derivatization processes must also be done in tubes or vials closed with
such device.
Dr C. Leray

Jodie a écrit :

> Hi everyone,
>
> I have a septa / liner / insert query.
> What does everyone use in their sample vials ?
>
> We have had a fairly random selection in our lab
> (plastic, teflon/rubber, and some mystery ones)
> and I think I might treat myself to some new
> ones as those that I've been using don't seem to
> survive a silylation reaction and I'm getting
> 'muck' in my vial, which is showing up on my
> GC.
>
> Any recommendations or "avoid these ones" ?
>
> Is Teflon the liner of choice ? Does it depend
> on which reaction you might be performing, i.e.
> would you use a different liner for storing lipids
> in solvent from that used for enzymatic or
> derivatisation reactions ?
>
> Thanks
> Jo
>
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