Hello:

does somebody have a working HPLC method to analyze
sphingomyelin and it's derivatives (notably
ceramide?). We have a very sensitive ELSD detector, and
several normal- and reversed-phase columns. I could not find a
decent method on medline.

I also would consider using thin-layer chromatography, provided
there is a method to quantify non-phosphate containing lipids.
Maybe somebody knows how to derivatise ceramide? Any help
would be greatly appreciated!


Erik Eckhardt, Ph.D.