Hello, Ruben!
I know nothing about electrophoretic methods for lipids. But I think
it possible to use lipids extraction and then determination of
lipid's phosphorus. If you need not common PL then use TLC and
different detection of fraction PL (lipid's phosphorus or
densitometry).
See also:
1. Alvarez JG, Slomovic B, Ludmir J.
Analysis of dipalmitoyl phosphatidylcholine in amniotic fluid by
enzymatic hydrolysis and high-performance thin-layer chromatography
reflectance spectrodensitometry.
J Chromatogr B Biomed Appl. 1995 Mar 10;665(1):79-87.
2. Ruiz-Budria J, Fabre-Gonzalez E, Higueras MT, Serrano-Ostariz JL.
Amniotic fluid phospholipids. New predictive values of L/S and PG/S
ratios.
Rev Esp Fisiol. 1995 Mar;51(1):17-21.

Search MEDLINE and you will find many articles by this theme.
Best wishes.
Gennadiy.
--- In lipids@e..., rcortez@c... wrote:
> Hello from Tepui's Land: VENEZUELA!
>
> I'm looking for a method to detect Phospholipids in Human
> Amniotic Fluid by Chromatographic or Electrophoretic method.
>
> Thanks in advance,
>
> Ruben Cortez