Hello,
I am very grateful for your answer. But I interest not only solvents
in which lipids are most soluble. I interest lipid's solubility in
different solvents (for instance: water, hexane, heptane,
ethanol...and so on). And also I interest lipid's solubility in other
lipids. In which quantity do they mix up whithout form separate phase?
Thanks.
I hope you understand my awful English.

--- In lipids@e..., "Jodie" <spbcjsb@i...> wrote:
> Hi,
>
> A lipid mixture (all different types of lipids) is soluble
> in chloroform mixed with either methanol or isopropanol, the
> ratio doesn't have to be exact, but most people seem to use
> 2:1 (ie. two parts chloroform to one part methanol).
>
> If you are interested in which classes of lipids are most
> soluble in which solvents types, the following table comes
> from the list of solvents used to fractionate lipid classes
> from solid phase extraction columns (aminopropyl-, or diol-
> bonded columns) [method adapted from Kaluzny 1985].
>
> Whole lipids placed on first column
> Neutral lipids Chloroform: Isopropanol [2:1]
>
> (neutral lipid fraction is dried and redissolved in hexane, then
> placed onto second column >>> )
>
> (meanwhile the first column is extracted with...)
> Free fatty acids 2% acetic acid in diethyl ether
> Phospholipids Methanol
>
> ( >>> neutral lipid on second column is then fractionated)
> Cholesteryl esters Hexane
> Triacylglycerols Hexane: dichloromethane: diethyl ether
> [89 : 10 : 1]
> Cholesterol Hexane : Ethyl acetate [95 : 5]
> Diacylglycerols Hexane : Ethyl acetate [85 :15]
> Monacylglycerols Chloroform : Methanol [2:1]
>
> Neutral lipids are more soluble in Chloroform: isopropanol than they
> are in pure hexane, but for this extraction procedure they are
> required to be in hexane.
>
> For individual compounds, you can check the Sigma website for
> solubility information (they give amounts as well, eg. "1mg / 10ml
> produces a clear solution") and also the Merck Index.
>
> The second question of solubility of lipids within lipids is
> something I don't have much experience of, and can be complicated
> by the different types of fatty acids making up the compound.
>
> For example, butterfat triglycerides contain more saturated fatty
> acids compared with olive oil triglycerides - thus in this case,
> triglycerides are not particularly soluble in triglycerides !
> Until you heat them.
>
> I can't imagine that triglycerides are soluble in cholesterol
> unless the triglycerides had very short chains (the longer chain
> ones behave as very non-polar, whereas cholesterol is more
> amphipathic). I'm really not sure though.
>
> For more information, contact some of the lipid companies (some
> contact details are available in the Bookmarks page at the
> website) as they routinely prepare and purify lipids.
>
> Hope that helps
> Jo
> http://groups.yahoo.com/group/lipids/links