Dear Group,
Greetings! I am wondering if anyone has compared the efficiency of
different extraction procedures on recovering polyunsaturated fatty acids.
I have tried several common methods (Folch, Dole, etc.) and recovered nearly
100% of palmitate or oleate, but found such fatty acids as 20:5 and 22:6 to
have poor recovery (70% at best).

I became aware of this problem when I tried to measure acid-soluble products
in some in vitro incubations with a variety of different radiolabeled long
chain fatty acids. What I found is that the acid precipitation of the fatty
acid/protein complex was not the same between fatty acids. So now I have
media with radiolabeled fatty acids, which are contaminating my acid-soluble
products measurements. Any thoughts on how to separate the two or
effectively extract PUFA would be greatly appreciated.

Best regards,

Doug

Doug Mashek
Graduate Research Assistant
Department of Dairy Science
University of Wisconsin-Madison
email: dgmashek@...
Ph: (608) 263-3497
Fax: (608) 263-9412

948 Animal Sciences Bldg.
1675 Observatory Drive
Madison, WI 53706-1284




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