Your observation is not clear. In what medium have you
made the fatty acid extraction ? is it in the presence
of cells or not ?
The recovery of fatty acids is improved in using an
acid medium.
Your results are curious and it seems that something
is wrong in your procedure. You can follow one of the
various procedures described in the website
www.cyberlipid.org

Best regards
C. Leray


--- Doug Mashek <dgmashek@...> a écrit :
> Dear Group,
> Greetings! I am wondering if anyone has compared
> the efficiency of
> different extraction procedures on recovering
> polyunsaturated fatty acids.
> I have tried several common methods (Folch, Dole,
> etc.) and recovered nearly
> 100% of palmitate or oleate, but found such fatty
> acids as 20:5 and 22:6 to
> have poor recovery (70% at best).
>
> I became aware of this problem when I tried to
> measure acid-soluble products
> in some in vitro incubations with a variety of
> different radiolabeled long
> chain fatty acids. What I found is that the acid
> precipitation of the fatty
> acid/protein complex was not the same between fatty
> acids. So now I have
> media with radiolabeled fatty acids, which are
> contaminating my acid-soluble
> products measurements. Any thoughts on how to
> separate the two or
> effectively extract PUFA would be greatly
> appreciated.
>
> Best regards,
>
> Doug
>
> Doug Mashek
> Graduate Research Assistant
> Department of Dairy Science
> University of Wisconsin-Madison
> email: dgmashek@...
> Ph: (608) 263-3497
> Fax: (608) 263-9412
>
> 948 Animal Sciences Bldg.
> 1675 Observatory Drive
> Madison, WI 53706-1284
>
>
>
>
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>
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