--- Claude Leray <clleray@...> a écrit : > Your
observation is not clear. In what medium have
> you
> made the fatty acid extraction ? is it in the
> presence
> of cells or not ?
> The recovery of fatty acids is improved in using an
> acid medium.
> Your results are curious and it seems that something
> is wrong in your procedure. You can follow one of
> the
> various procedures described in the website
> www.cyberlipid.org
>
> Best regards
> C. Leray
>
>
> --- Doug Mashek <dgmashek@...> a
> écrit :
> > Dear Group,
> > Greetings! I am wondering if anyone has compared
> > the efficiency of
> > different extraction procedures on recovering
> > polyunsaturated fatty acids.
> > I have tried several common methods (Folch, Dole,
> > etc.) and recovered nearly
> > 100% of palmitate or oleate, but found such fatty
> > acids as 20:5 and 22:6 to
> > have poor recovery (70% at best).
> >
> > I became aware of this problem when I tried to
> > measure acid-soluble products
> > in some in vitro incubations with a variety of
> > different radiolabeled long
> > chain fatty acids. What I found is that the acid
> > precipitation of the fatty
> > acid/protein complex was not the same between
> fatty
> > acids. So now I have
> > media with radiolabeled fatty acids, which are
> > contaminating my acid-soluble
> > products measurements. Any thoughts on how to
> > separate the two or
> > effectively extract PUFA would be greatly
> > appreciated.
> >
> > Best regards,
> >
> > Doug
> >
> > Doug Mashek
> > Graduate Research Assistant
> > Department of Dairy Science
> > University of Wisconsin-Madison
> > email: dgmashek@...
> > Ph: (608) 263-3497
> > Fax: (608) 263-9412
> >
> > 948 Animal Sciences Bldg.
> > 1675 Observatory Drive
> > Madison, WI 53706-1284
> >
> >
> >
> >
> > [Non-text portions of this message have been
> > removed]
> >
> >
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> >
> >
> >
>
>
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