You have globally two solutions :
1- saponify the lipid extract (as described in
cyberlipid.org)in the presence of antioxidants and
separate the tocopherols by reversed-phase
chromatography.

2- separate the crude extract, even directly the oil,
by normal phase chromatography

You may look at the following references:
Gimeno E et al J Chromatography A 2000, 881, 251-4

Ruperez FJ et al J Chromatography A 2001, 935, 45-69

Best regards
Claude Leray






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