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[Non-text portions of this message have been removed]
You might have significant oxidation of your samples, especially of unsaturated
fats, and
there are plenty of those in fish oil. I would put the sample in an air-tight
container,
preferably glass, and blow argon over it, or if you don't have argon, you could
use
nitrogen gas. Otherwise you could put the samples in food-grade plastic bags
which you
then vacuum-seal.
EE
--- In lipids@..., Claude Leray <clleray@...> wrote:
>
>
> Hello
> There is no problem for the transport of lyophilized
> samples even for some days in the darkness. Don't
> forget to wet the sample before lipid extraction
> because lipid recovery is not important with dry
> samples.
> Cordially
> Dr Claude Leray
>
>
> --- apostolos_koussoroplis
> <apostolos_koussoroplis@...> a écrit :
>
> > Hello,
> >
> > I'm a research student wornikg on fatty acid
> > biomarkers in aquatic food
> > webs and in particular compound specific stable
> > isotope analysis.
> > Since my lyophilized samples will have to travel by
> > plane mostly (about
> > 12h) and I doubt they accept liquid Nitrogen on
> > board, I'm looking for
> > methods to protect them from alteration....
> > Any suggestions?
> > I have been told that lyophilised samples could
> > travel by post
> >
> >
> >
>
>
>
>
>
>
>
> ___________________________________________________________________________
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> Profitez des connaissances, des opinions et des expériences des internautes
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Hello
There is no problem for the transport of lyophilized
samples even for some days in the darkness. Don't
forget to wet the sample before lipid extraction
because lipid recovery is not important with dry
samples.
Cordially
Dr Claude Leray
--- apostolos_koussoroplis
<apostolos_koussoroplis@...> a écrit :
> Hello,
>
> I'm a research student wornikg on fatty acid
> biomarkers in aquatic food
> webs and in particular compound specific stable
> isotope analysis.
> Since my lyophilized samples will have to travel by
> plane mostly (about
> 12h) and I doubt they accept liquid Nitrogen on
> board, I'm looking for
> methods to protect them from alteration....
> Any suggestions?
> I have been told that lyophilised samples could
> travel by post
>
>
>
___________________________________________________________________________
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Hello,
I'm a research student wornikg on fatty acid biomarkers in aquatic food
webs and in particular compound specific stable isotope analysis.
Since my lyophilized samples will have to travel by plane mostly (about
12h) and I doubt they accept liquid Nitrogen on board, I'm looking for
methods to protect them from alteration....
Any suggestions?
I have been told that lyophilised samples could travel by post
thanx alot vaijnath lad....tc.
vaijnath lad <vaijnath.lad@...> wrote: Hellow There,
Please visit following websites , you will get lot of stuff on avocado.
http://www.avocadosource.com/http://www.avocado.org/
regards,
vaijnath lad
On 1 Jun 2006 10:24:44 -0000, lipids@... <
lipids@...> wrote:
>
> There is 1 message in this issue.
>
> Topics in this digest:
>
> 1. literature review
> From: "dhev" <dhev_cacti@...>
>
>
> ________________________________________________________________________
> ________________________________________________________________________
>
> Message: 1
> Date: Wed, 31 May 2006 13:52:18 -0000
> From: "dhev" <dhev_cacti@...>
> Subject: literature review
>
> where can i find literature reviews regarding lipid analysis in
> avocado?..thank u dr claude..
>
>
>
>
>
> ________________________________________________________________________
> ________________________________________________________________________
>
>
> Lipid Links page: http://groups.yahoo.com/group/lipids/links
> Unsubscribing ? mailto:lipids-unsubscribe@...
> Questions ? Please contact Jo at mailto:spbcjsb@...
>
>
> ------------------------------------------------------------------------
> Yahoo! Groups Links
>
>
>
>
> ------------------------------------------------------------------------
>
>
>
>
>
--
With Best Regards,
Vaijnath Lad
[Non-text portions of this message have been removed]
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[Non-text portions of this message have been removed]
Hellow There,
Please visit following websites , you will get lot of stuff on avocado.
http://www.avocadosource.com/http://www.avocado.org/
regards,
vaijnath lad
On 1 Jun 2006 10:24:44 -0000, lipids@... <
lipids@...> wrote:
>
> There is 1 message in this issue.
>
> Topics in this digest:
>
> 1. literature review
> From: "dhev" <dhev_cacti@...>
>
>
> ________________________________________________________________________
> ________________________________________________________________________
>
> Message: 1
> Date: Wed, 31 May 2006 13:52:18 -0000
> From: "dhev" <dhev_cacti@...>
> Subject: literature review
>
> where can i find literature reviews regarding lipid analysis in
> avocado?..thank u dr claude..
>
>
>
>
>
> ________________________________________________________________________
> ________________________________________________________________________
>
>
> Lipid Links page: http://groups.yahoo.com/group/lipids/links
> Unsubscribing ? mailto:lipids-unsubscribe@...
> Questions ? Please contact Jo at mailto:spbcjsb@...
>
>
> ------------------------------------------------------------------------
> Yahoo! Groups Links
>
>
>
>
> ------------------------------------------------------------------------
>
>
>
>
>
--
With Best Regards,
Vaijnath Lad
[Non-text portions of this message have been removed]
Dear Dhevaki Dhev,
Thank you very much for your kind mail.I am sorry to say that
i am also a post graduate student doing research on Liposomes. I didnt have
clear idea regarding your project.
sorry but unable to help you.If you want i can send some articles for you.
with regards
mallesh
On 5/24/06, dhevaki dhev <dhev_cacti@...> wrote:
>
> im doing research in final year.characterization of lipids in
> avocado.what are the proper method and techniques that i can use to
> conduct the experiment?what are the chemical stuffs and equipments
> needed?thank u in advance for your kind attention.
>
>
> ---------------------------------
> All new Yahoo! Mail "The new Interface is stunning in its simplicity and
> ease of use." - PC Magazine
>
> [Non-text portions of this message have been removed]
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>
>
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[Non-text portions of this message have been removed]
im doing research in final year.characterization of lipids in avocado.what are
the proper method and techniques that i can use to conduct the experiment?what
are the chemical stuffs and equipments needed?thank u in advance for your kind
attention.
---------------------------------
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[Non-text portions of this message have been removed]
... Dear all,
I am not very familiar with conventional techniques in
glycosphingolipid science, therefore pardon these naive questions:
1- what is your experience in coating whatever type of surface with
a purified GSL (yieldwise). I need to perform binding experiments in
order to fish out a ligand for the forssman GSL (could be protein or
other GSL). I have tried various types of 96-well plates. After
several washings I can still detect the forssman molecule with a
monoclonal antibody but the signal (ELISA type of detection) is very
weak. Starting in the microgram range at the time of coating I find
myself with something that is probably in the nanogram range at the
time of detection. Is there a way of increasing the GSL molecular
density (per unit surface of substratum?). What type of material
would you recommand using for high yield coating?
2- PDMP, PPMP and PPPP are widely described as glycosyl ceramide
transferase inhibitors. What is known exactely regarding their
inhibitoty activity on galactosyl ceramide transferase?
Reproducibly, PPPP failed to exhibit any cell/cell binding activity
whatsoever in our particular model where PDMP, PPMP and even
Fumonisin B1 produce a substantial reduction of binding (WITHOUT
APPARANT APOPTOSIS OR CELL DAMAGE except maybe for a notable
reduction in proliferation pace). I have seen that PPPP would be
relevant for Fabry disease treatment as illustrated by its capacity
to reduce Gb3 levels... has anybody ever checked galabiosyl ceramide
levels as well?
I will be waiting eagerly for your input and comments.
Many thanks in advance
Paola
Dear Dr. Leray
Thank you very much for your valuable
comments.Congrats for the ciberlipid center web-page;
it´s a excellent resourse for those needing
information about lipids and their analyisis.
Cordially, G. Obal.
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Hi friend,
The new spot developed may be a Fatty alcohol, why not
silylate it and see whether it is an alcohol or not.
gonzalo obal <gonchi3001@...> wrote:
Dear Friends,
The analysis by HPTLC of a parasite lipid extract
shows a band migratin between FAMEs (used as a
standard of migration) and sterol esters. The
migration pattern (in hexane/diethylether/acetic acic
80/20/1)is follows: TAGs - FAMEs - Unknown - Sterol
sters - Hidrocarbons; all bands resolved clearly. I
have checked a lot of information (generally
considered comprehensive) bu i could found any
reference about the kind of lipid class it could be.
Generally, available information reports TAGs, FAMES,
and SS, but no data of lipid classes migrating
slightly further than FAMEs. This lipid component is
preseumed to be biologically relevant and not a
contamintant: it is seen in several independent
extracts but not in controls. Has anyone any idea,
about it? Thanks in advance.
__________________________________________________
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[Non-text portions of this message have been removed]
Dear Gonzalo Obal,
several substances can migrate between FAMEs and
sterol esters.
It's possible you have isolated alkyl diacylglycerols
(with one or two alkyl groups) and also ester waxes. A
saponification step before the TLC could bring some
information, similarly on the substances separated in
the first migration and separated again in a second
migration step. Any modification in the migration
pattern may throw some light on your problem.
Cordially
Claude Leray
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dear Claude Leray <clleray@...>
IS THERE ANY METHOD FOR THE EXTRACTION AND PURIFICATION OF GLYCOSIDES FROM THE
LEAVES OF MEDICINAL PLANTS ? PLZZZZ EXPLAIN... CIAO
SCOTTDOLAN
---------------------------------
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[Non-text portions of this message have been removed]
Dear Friends,
The analysis by HPTLC of a parasite lipid extract
shows a band migratin between FAMEs (used as a
standard of migration) and sterol esters. The
migration pattern (in hexane/diethylether/acetic acic
80/20/1)is follows: TAGs - FAMEs - Unknown - Sterol
sters - Hidrocarbons; all bands resolved clearly. I
have checked a lot of information (generally
considered comprehensive) bu i could found any
reference about the kind of lipid class it could be.
Generally, available information reports TAGs, FAMES,
and SS, but no data of lipid classes migrating
slightly further than FAMEs. This lipid component is
preseumed to be biologically relevant and not a
contamintant: it is seen in several independent
extracts but not in controls. Has anyone any idea,
about it? Thanks in advance.
__________________________________________________
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Dear Nutan,
your procedure is correct if you follow some necessary
precautions i.e. an efficient extraction procedure and
a precise protocole for drying the extract.
You will find a classic and recommended description in
that web page :
http://www.cyberlipid.org/extract/extr0005.htm#1
Other extraction procedures may be used but the Folch
procedure is the best known among lipidologists.
Cordially
Dr Claude Leray
--- lipid sls <lipid_sls@...> a écrit :
> Dear Friends,
>
> can anyone suggest me any method to estimate how
> much total lipid(quantity)is present in an extract
> from a animal tissue. actually i was doing it on dry
> wieght basis but not sure whether it is correct or
> not. any internationally accepted method is
> wellcome.
>
> nutan
>
>
>
>
>
>
>
__________________________________________________________
>
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>
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>
>
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Dear Friends,
can anyone suggest me any method to estimate how
much total lipid(quantity)is present in an extract
from a animal tissue. actually i was doing it on dry
wieght basis but not sure whether it is correct or
not. any internationally accepted method is wellcome.
nutan
__________________________________________________________
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Dear All,
While surfing I came across this beautifull and knowledgable group,
and I immediately joined this.
I would like to give little intro of mine.
I am Vaijnath Lad, a B.Tech. oils graduate from india and working in
cosmetics industry from last 4 years.
Currently working with IFSC a danish company as Technical Manager -
Cosmetics for their south east operation headed in india.
I am really impressed by joining this group and would like to have many
friends on this group to share knowledge.
Thanking you.
regards,
vaijnath lad
Hi Max,
the fat precipitated may be a triglyceride containing saturated fatty
acids as major fractions. Bcaz, we have come across a similar type of problem
where we found that a triglyceride rich in saturated fatty acid was precipitated
out.
max51692000 <max51692000@...> wrote:
Dear collegues,
Does anybody know anything about the thechnique called: "freezing-out
technique". I encountered this technique in many papers but found no
references on the literature.
Basically, when I stick my acetone extract of a freeze-dried tissue
(or a wet tissue) on the refridgerator (-20 degrees C), I see a fat
precipitation - floculation appearing on my tubes.
Any idea which fat precipitate? Triglycerides? Polar fat?
And what is the physico-chemical process taking place on my tube at
this temperature?
Any help, references on that topic would be appreciated.
Maxime
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[Non-text portions of this message have been removed]
Dear collegues,
Does anybody know anything about the thechnique called: "freezing-out
technique". I encountered this technique in many papers but found no
references on the literature.
Basically, when I stick my acetone extract of a freeze-dried tissue
(or a wet tissue) on the refridgerator (-20 degrees C), I see a fat
precipitation - floculation appearing on my tubes.
Any idea which fat precipitate? Triglycerides? Polar fat?
And what is the physico-chemical process taking place on my tube at
this temperature?
Any help, references on that topic would be appreciated.
Maxime
Hi everyone. I´m working with a lipid binding protein
that shows in SDS-PAGE, under non-reducing and
reducing conditions, a ladder-type pattern of
subunits. Some lines of evidence indicates to me that
the lipid-protein motifs are organized in a hydrophic
core/polar outer shield, similar to that described for
animal serum lipoproteins. Moreover, some previous
observations has shown that this structural
organization is not significantly altered even in the
presence of 6M urea.
Then my specific question is: if the native protein is
treated in order to be analized by denaturing PAGE
(non-red. or red.), (that is heated at 105 degrees for
5 min in the presence of SDS) and a band corresponding
to a particular subunit is scissed from the gel and
the protein is then recovered by electroelution, ¿is
it possible that some lipids could still be associated
to the protein? If its true, ¿are they binded in a
negligible or significantly amount? ¿or are all the
lipidic components removed during heating in the
presence of detergent? ¿is it possible that the lipids
could not migrate during electrophoresis, so they stay
at the wells on the stacking gel? I am sorry that i
couldn´t make a shorter exposition, but i´ll be very
gratefully. It´s a recent idea that come to my mind
and i could not get a confident answer. Thanks in
advance. G. Obal
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Dear Shiva,
there are a lot of references on glycolipids replacing
the true lipids to reduce calorie intake.
I suggest you to look at the following page :
http://www.caloriecontrol.org/frgloss.html
and to search in Google the terms which correspond to
those acting as reduced calorie fats.
Sincerely yours
Dr Claude Leray
--- shiva shanker <chemtinku@...> a écrit :
> please send me any literature available on
> GLYCOLIPIDS and Reduced Calorie Fats
>
>
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>
>
>
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[Non-text portions of this message have been removed]
Hi. I would like to ask some questions regarding lipids:
1.) Rationalize the fact that glycerophosphatides can be extracted by
hexane.
2.) Why is there a need for glycerophosphatides to be stored in
methanol - choloroform mixture?
3.) Why are sphingosines extracted with boiling ethanol?
4.) What are the structural formulas of sphingosine phosphatide,
sphingosine glycoside and glycerophosphatides?
Hello again,
Another question regarding carotenoids... (by the way thanks a lot Dr.
Leray, both for your excellent and comprehensive website and also for
this discussion group).
I've seen in a paper that Kow values (octanol-water partitioning
coeficient) were given for carotenoids (and lipid soluble vitamins) in
the range of 12-14 (values estimated from the model KOWWIN developped
by Syracuse university, NY). Is it a common practice in lipid sciences
to express the hydrophobicity of a compound using Kow values?
I am wondering at first because I am an environmental scientist and we
use this chemodynamic value to express the behaviour of an
anthropogenic contaminant such as PCBs, PAHs... also because as far
as I know a Kow value of more than 8-9 is out of the "experimental
scale"...
Thanks
Maxime
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