Hello everybody, I am not very confident with the furtherance of previous contacts I have made with lipid specialists... this site might then be very useful to...
Hello, I can give you the Rf values I had with the first solvent system according to the Heape's method with a 7.5 cm running distance on HPTLC plates : PE:...
Dear Lipid Fraternity, I am working with a oil processing company in India We have options with us to use either "Solvent Extracted Sunflower oil "or "Crude...
Hi, I am experiencing some difficulty in extracting non-polar lipids. I was wondering if any one out there knows of any protocols (in websites or journal...
Hi, thanks for replying. The lipids are taken from Mycobacterium bovis cells http://en.wikipedia.org/wiki/Mycobacterium_bovis Non-polar lipids that we want to...
I suggest you to apply the extraction recipe adapted for bacteria and described in the following paper : Lewis T et al., J Microbiol meth 2000, 43, 107-116. ...
Hie, bonjour I'd like to know if anyone has suggestions on how to seperate xanthophyll carotenoids (and their respective esters) from a lipid matrix. I am...
Hello The best way to isolate xanthophylls and chlorophylls from a lipid mixture is to use a liquid partition as dsecribed by Minguez-Mosquera for oils (J Am...
Dear Dr.Claude wat `s the purpose of using the ratio 2:1 with benzene:methanol or 2:1 with hexane:methanol in the extraction of pigments from leaf .PLZZ...
Hello again, Another question regarding carotenoids... (by the way thanks a lot Dr. Leray, both for your excellent and comprehensive website and also for this...
Hi. I would like to ask some questions regarding lipids: 1.) Rationalize the fact that glycerophosphatides can be extracted by hexane. 2.) Why is there a need...
please send me any literature available on GLYCOLIPIDS and Reduced Calorie Fats ... Check out Yahoo! India Rakhi Special for Rakhi shopping, contests and lots...
Dear Shiva, there are a lot of references on glycolipids replacing the true lipids to reduce calorie intake. I suggest you to look at the following page : ...
Hi everyone. I´m working with a lipid binding protein that shows in SDS-PAGE, under non-reducing and reducing conditions, a ladder-type pattern of subunits....
Dear collegues, Does anybody know anything about the thechnique called: "freezing-out technique". I encountered this technique in many papers but found no ...
Hi Max, the fat precipitated may be a triglyceride containing saturated fatty acids as major fractions. Bcaz, we have come across a similar type of problem...
Dear All, While surfing I came across this beautifull and knowledgable group, and I immediately joined this. I would like to give little intro of mine. I am...
Dear Friends, can anyone suggest me any method to estimate how much total lipid(quantity)is present in an extract from a animal tissue. actually i was doing it...
Dear Nutan, your procedure is correct if you follow some necessary precautions i.e. an efficient extraction procedure and a precise protocole for drying the...
Dear Friends, The analysis by HPTLC of a parasite lipid extract shows a band migratin between FAMEs (used as a standard of migration) and sterol esters. The ...
Dear Gonzalo Obal, several substances can migrate between FAMEs and sterol esters. It's possible you have isolated alkyl diacylglycerols (with one or two alkyl...
