Did anyone from the list go to the B2 conference earlier in the year in New Mexico ? I'd love to hear how it went as it looked very interesting, not to...
Dear all! I would like to know if any of you know a desaturase inhibitor, that can be used to manipulate the membrane lipid composition of bacteria. Best...
Yes there are several synthetic inhibitors of fatty acid desaturases, you will find a lot of references in screening PuMed with Fatty, desaturase and inhibitor...
You have globally two solutions : 1- saponify the lipid extract (as described in cyberlipid.org)in the presence of antioxidants and separate the tocopherols by...
Dear colleagues: I have two questions related to quantitative extraction of lipids (from complex systems). First, Folch method sets a the final proportion...
Dear Mr Herrera Your question about the volume of the lower phase has no practical importance with the lipid extraction process. You must remove the upper or...
does somebody know a good (commercial) source of or (biochemical) method to obtain radiolabelled 4-hydroxysphinganine (phytosphingosine) ? Paul P. Van...
dears I try to separate some plant sterols from serum by GC i facing a problem where cholesterol was load the colum, Can you help me? Where can I get...
To the best of my knowledge, nobody has reported the purification of a phytosterol fraction devoided of cholesterol. The plant sterols are chemically too close...
Dear all: Does anyone know if the use of FEP tubes during the extraction of lipids with chloroform and methanol cause problems in subsequent FAME analysis by ...
Dear Dr Herrera FEP tubes are made of propylene and any trace of monomers can spoil the preparation if you dont fractionate the extract by chromatography. The...
Dear All, For anyone interested, here in the Department of Cell Biology and Oncology at the Consorzio Mario Negri Sud in Italy we are starting a "recruitment...
Hello, I was referred to this group by the person who runs Cyberlipids.com, and so I hope I'm not violating any protocols by posting a position announcement...
Dear all: I may need to deoxygenate solvents and some aqueous solutions to determine the conten o fhydroperoxides in my samples by purging with nitrogen. I...
I would suggest that you use vaccum de-air and break with nitrogen at least three time before bubbling with nitrgoen. Also, if you may be better off to remove ...
Several device may be found to deoxygenate solvents. The best for HPLC is to use an on line vacuum instrument able to manage 4 lines of solvents. With the help...
hi, Is there any prepacked silica column that can be used for the separation of the polar FA from the non-polar FA? FOr the microbial community analysis(a...
To the best of my knowledge, it is not possible readily to isolate by HPLC polar and non-polar fatty acids. But, you can fractionate a complex pool of FAME by...
I request to refractive index as function of wavelenght or energy (Real and Imaginary) of: PC: phosphatidylcholine Ethly-DOPC: ethyl-dioleylphosphatidylcholine...
I'd like to ask whether anyone has experience using a PVA-Sil column to analyze for phosphatidylserine (PS) with an HPLC-ELSD system, or, alternatively, knows...
I've only just found out about the Glycolipids discussion / mini- symposium which is taking place in London, UK this Wednesday and Thursday at the Royal...
Dear all: Regarding the thiocyanate method to quantify hydroperoxides, different authors use different times to take readings. It seems clear that in some ...
Dear Dr Herrera The thiocyanate method is very sensitive to oxygen thus you must deoxygenate the lipid extract and the reagent before the reaction. I advise...
Dear colleagues, I need a DETAILED laboratory protocol for synthesis of analytical amounts of conjugated linoleic acid mixture. Normally it is obtained by...
For anyone who might be interested, we'll be running our fourth FEBS Advanced Course next June. This time the emphasis has changed slightly, and it is titled...
Hi I hope some one can help me. How do you calculate how many different triglycerides can be made from a glycerol and three fatty acids. How many triglycerides...
If I understand correctly, it's a matter of the number of permutations of three different fatty acids, A, B, and C: AAA AAB AAC ABA ABB ABC ACA ACB ACC BAA BAB...
I was loking for an expriment conducted by Rothman and Kennedy using gram pos bacteria, that sheds some light on movement of phospholipids in membranes. I...
Hi Angela, The Rothman & Kennedy paper appears to be from 1977 so that probably explains its absence on the internet. You might find these to be quite helpful...
